...1 Capping System C-SCCS1710_美国lucigen_技术参数_价格-仪器网

来自 : 发布时间：2024-05-03

ScriptCap™ Cap 1 Capping System C-SCCS1710产品简介：北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。ScriptCap™ Cap 1 Capping System C-SCCS1710品牌介绍：CELLSCRIPT专注于再生医学，细胞再生学以及细胞疗法。细胞、组织或器官的移植ZL及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。ScriptCap™ Cap 1 Capping System C-SCCS1710规格详情名称：ScriptCap™ Cap 1 Capping System分类：Post-Transcriptional Capping货号：C-SCCS1710规格：10 reactions产品详情：For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2\'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.供应商：中北林格/p>产地：us发货地：北京运输方式：送货上门/顺丰是否现货：期货ScriptCap™ Cap 1 Capping System C-SCCS1710产品详情A. Synthesis of Cap 0 RNA from Uncapped RNA1. The protocol below was designed to build a cap 1 structure on 5\' end of 50-60 µg of uncapped RNA. If a cap 0 structure is desired, replace the ScriptCap 2\'-O-Methyltransferase in Step 3 with an equivalent volume of RNase-Free Water. Combine the following reagents:Standard ScriptCap Cap 1 Capping Reaction (step 1)ComponentAmountRNase-Free Waterx µlIn vitro transcribed RNA, 50-60 µg≤67 µTotal Volume67 µl2. Incubate at 65o C for 5-10 minutes, then transfer to ice.3. While the heat-denatured RNA is coo领 on ice, prepare a \"Cocktail” of the following reaction components together in a separate tube.Standard ScriptCap Cap 1 Capping Reaction (step 3)ComponentAmount10X ScriptCap Capping Buffer10 µl10 mM GTP10 µl20 mM SAM2.5 µlScriptGuard RNase Inhibitor2.5 µlScriptCap 2\'-O-Methyltransferase (100 U/µl)4 µlTotal Volume29 µl4. Just prior to starting the reaction, add the ScriptCap Capping Enzyme to the Cocktail from Step 3 and then combine this with the uncapped RNA solution from Step 1.Standard ScriptCap Cap 1 Capping Reaction (step 4)ComponentAmountCocktailed reaction components (from step 3)29 µlScriptCap Capping Enzyme (10 U/µl)4 µlHeat-denatured RNA (from step 1)67 µlTotal Reaction Volume100 µl5. Incubate at 37o C for 30 minutes.6. The Capped RNA can now be purified, or it can be 3\' polyadenylated without purification by adding the reaction mixture directly to an A-Plus Poly(A) Polymerase Tai领 Kit reaction (sold separately).ScriptCap™ Cap 1 Capping System Contents (10 Reactions)Kit ComponentReagent VolumeScriptCap™ Capping Enzyme, 10 U/µl (red cap)40 µlScriptCap™ 2\'-O-Methyltransferase, 100 U/µl (blue cap)40 µl10X ScriptCap™ Capping Buffer. 0.5 M Tris-HCl, pH 8.0, 60 mM KCl and 12.5 mM MgCl2100 µl10 mM GTP Solution100 µl20 mM S-adenosyl-methionine (SAM)25 µlScriptGuard™ RNase Inhibitor, 40 U/µl25 µlRNase-Free Water1.4 ml订购注意事项 如果您是初次订货，请订货时提供如下信息： 1、品名或货号、包装规格、数量、生产厂家等 2、订货人姓名、单位、 4、付款单位或开票名称 5、确认开票规格、付票名称 如果已购买过cellscript试剂盒，仅需提供货号或品名、包装规格、数量、生产厂家即可。关于发货： 1、部分产品备有现货一般下午三点前完成付款当天发出，超过截止时间造成当日不能发货的将于次日安排发货。 2、-80℃保存的产品一般次日发出，需要预定干冰发货。 3、期货产品货期3周左右，每周四向国外下订单，周四下午三点前截单。 4、请办理完货款后，将收据、底单等连同收货人的地/址、姓/名、电/话等信息通过Q/Q,Email等形式发送通知我们。ScriptCap™ Cap 1 Capping System C-SCCS1710 关键词：ScriptCap™ Cap 1 Capping Systemcellscript ScriptCap™ Cap 1 Capping Systemcellscript总代理 ScriptCap™ Cap 1 Capping Systemcellscript总代理中北林格 ScriptCap™ Cap 1 Capping Systemcellscript C-SCCS1710 10 reactionscellscript总代理 C-SCCS1710 10 reactionscellscript总代理中北林格 C-SCCS1710 10 reactionscellscript Post-Transcriptional Capping C-SCCS1710cellscript总代理 Post-Transcriptional Capping C-SCCS1710cellscript总代理中北林格 Post-Transcriptional Capping C-SCCS1710对此产品有兴趣，可联系中北林格获取更多详细信息。